Los extremos de las proteínas cobran importancia en estudios de proteómica masiva

Investigadores de la Universidad de Gante (Bélgica) y de la Universitat Autònoma de Barcelona (UAB) han desarrollado un nuevo procedimiento para identificar los dos extremos de las moléculas de proteínas y su procesamiento-maduración en estudios de proteómica masiva ‘in vivo’ o ‘ex vivo’. El trabajo ha sido publicado en Nature Methods.

Hasta hace muy poco la mayoría de los estudios de proteómica se han centrado en las regiones internas de las proteínas o en su extremo N-terminal (-Nt), aquel que tiene un grupo amino, NH2, desde el que se empieza a contar la cadena lineal de aminoácidos enlazados de las proteínas. Esta focalización de estudios se ha debido a que los procedimientos y química para estudiar estas regiones N-terminal e internas son más fáciles y se han podido desarrollar antes.El nuevo método desarrollado permite ampliar estos estudios al extremo C-terminal de las proteínas (-Ct), aquel que tiene el grupo carboxilo, COOH, que constituye el final de las cadenas lineales de aminoácidos.

En adelante se podrá estudiar, por métodos masivos proteómicos, procesos de maduración de las proteínas que suelen involucrar cortes en su secuencia lineal, tanto a nivel interno como de sus dos extremos Nt- y -Ct. Esto permite evaluar la participación concreta de estas regiones en los procesos mencionados y en funciones como la generación, activación y desactivación de hormonas, los factores de crecimiento, los neuropéptidos, las enzimas, los receptores de membrana y otras muchas proteínas de importante acción biológica. La estrategia desarrollada se puede llevar a cabo ‘ex vivo’ (extractos celulares) o ‘in vivo’ (por transfección seguida de análisis proteómico). Además, por sí mismos, los extremos proteicos se suelen encontrar involucrados en funciones muy importantes, como localización y direccionamiento celular-tisular, inicio-afectación del plegamiento tridimensional, unión a otras biomoléculas y macro-estructuras y modificaciones químicas post-generación de proteínas, entre otros.

Fuente:www.diariomedico.com

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